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. 2019 Dec 6;8(12):1586. doi: 10.3390/cells8121586

Figure 3.

Figure 3

Biotin tagged UFC1 co-localized with Z-hAAT not wild-type hAAT in hepatocytes: (A) 10 µM UFC1 cultured with Z-hAAT hepatocytes for 24 h; (B) 10 µM biotin tagged UFC1 cultured with Z-hAAT hepatocytes for 24 h; (C) 10 µM UFC1 cultured with HepG2 cells for 24 h; and (D) 10 µM biotin tagged UFC1 cultured with HepG2 cells for 24 h. hAAT, green color (first antibody, rabbit anti-human AAT; second antibody, anti-rabbit IgG, Alexa Fluor 555). Biotin tagged UFC1, red color (Streptavidin-TMR). hAAT overlapping with biotin tagged UFC1, orange color.