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. 2019 Nov 28;8(12):1538. doi: 10.3390/cells8121538

Figure 6.

Figure 6

RTK inhibition abrogates RTK expression in NCI-H3122 R cells. (A) NCI-H3122 R cells were treated with afatinib or gefitinib. The expression levels of phospho-EGFR, EGFR, phospho-AKT S473, AKT, phospho-HER2, HER2, phospho-MEK S217/221, phospho-MEK S298, and MEK were assessed by Western blotting in NCI-H3122 R cells. β-Actin served as the loading control. (B) Basal expression of HIF-1α was evaluated with Western blotting. GAPDH served as the loading control (SEM, serum-free media; GM; growth media). (C) NCI-H3122 R cells were treated with afatinib of gefitinib. The expression levels of HIF-1α were evaluated with Western blotting in NCI-H3122 R cells. GAPDH served as the loading control. (D) Colony-forming assays were performed in NCI-H3122 R cells. Means with different letters (a, b, and c) indicate statistically significant differences (p < 0.05; N.S., not significant).