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. 2019 Nov 22;8(12):1495. doi: 10.3390/cells8121495

Figure 2.

Figure 2

Study of miR-33a-5p-targets by gain and loss function assays; hMSCs after 24 h of transfection with specific mimic and antimiR were analyzed for the gene expression modulation by qRT-PCR and protein Western blot analysis. (A) miR-33a-5p targets HMGA-2; (B) EMT markers: SNAIL, SLUG, TWIST, TGF-β; (C) Slug; (D) ALPL. Quantitative RT-PCR data are expressed as fold of change (FOI) in gene expression (2−ΔΔCt) that occurred in mimic or antimiR vs. scramble groups. OsteoImage assay was performed to quantify the in vitro mineralization nodules, and data are shown as relative fluorescence units (RFU,492/520 nm) (E) Western blot analysis for Runx-2 (F) protein was performed on total cell extract after miR-33a-5p and 3p over-expression or inhibition. Student t test: * p < 0.05, ** p < 0.005, *** p < 0.0005 between experimental group.