Table 1.
List of gene primers primers used to study gene expression profiling obtained by Qiagen or custom-made. Their expression was normalized to the β-actin housekeeping gene.
| Gene | Qiagen Primers QuantiTect Primer Assay | Catalog Number |
|---|---|---|
| RUNX-2 “Runt-related transcription factor 2” | Hs_RUNX2_1_SG | QT00020517 |
| ALPL “Alkaline phosphatase” | Hs_ALPL_1_SG | QT00012957 |
| BGLAP “bone gamma-carboxyglutamic acid-containing protein” | Hs_BGLAP_1_SG | QT00232771 |
| SPP1 “Secreted Phosphoprotein 1” | Hs_SPP1_1_SG | QT01008798 |
| Gene | Custom Made Primers Forward | Custom Made Primers Reverse |
| SNAIL “Snail family transcriptional repressor 1” | GCGAGCTGCAGGACTCTAAT | CCCGCAATGGTCCACAAAAC |
| SLUG “Snail family transcriptional repressor 2” | CATGCCTGTCATACCACAAC | GGTGTCAGATGGAGGAGGG |
| TWIST “Twist family bHLH transcription factor 1” | CGGCCAGGTACATCGACTTC | CAGAGGTGTGAGGATGGTGC |
| TGF-B “Transforming growth factor beta 1” | TGGTGGAAACCCACAACGAA | ACACAGAGATCCGCAGTCCT |
| YAP “Yes associated protein 1” | CCTCGTTTTGCCATGAACCAG | GTTCTTGCTGTTTCAGCCGCAG |
| TAZ “WW domain containing transcription regulator” | TGGACCAAGTACATGAACCACC | TCCACAGCCGACTTGTTCTC |
| HMGA-2 “High mobility group AT-hook 2” | GCGCCTCAGAAGAGAGGAC | GTCTTCCCCTGGGTCTCTTAG |
| EGFR “Epidermal growth factor receptor” | CCTATGTGCAGAGGAATTATGATCTTT | CCACTGTGTTGAGGGCAATG |
| ERK-2 “Extracellular signal-regulated kinases -2” | GCGCTACACTAATCTCTCGT | CTGAGGTGCTGTGTCTTCAA |
| ERK-3 “Extracellular signal-regulated kinases -3” | GAATGGCAAATCTGCTCAATT | ACAGTCCTCCCCACCACTCA |
| Reference Gene | Forward | Reverse |
| ACTB “Beta-Actin” | ATCAAGATCATTGCTCCTCCTGA | CTGCTTGCTGATCCACATCTG |