Table 1.
Antibodies and staining conditions.
| Target | Tissue Section | Primary Antibody | Secondary Antibody | ||
|---|---|---|---|---|---|
| Antibody | Dilution | Antibody | Dilution | ||
| CYP3A | Frozen | Rabbit anti-CYP3A1 | 1:250 | Swine anti-rabbit | 1:20 |
| CYP1A | Frozen | Rat anti-CYP1A2 | 1:500 | Rabbit anti-rat IgG | 1:1000 |
| CYP2C | Frozen | Rat anti-CYP2C6 | 1:250 | Rabbit anti-rat IgG | 1:1000 |
| CYP2E1 | Frozen/ FFPE |
Rabbit anti-CYP2E1 | 1:100 | Swine anti-rabbit | 1:20 |
| GS | FFPE | Mouse anti-GS | 1:1000 | anti-mouse | 1:500 |
| Arginase1 | FFPE | Anti-arginase-1 antibody, rabbit monoclonal | 1:500 | Swine anti-rabbit | 1:20 |
| CPS1 | FFPE | Anti-CPS1 antibody—liver mitochondrial marker | 1:500 | Swine anti-rabbit | 1:20 |