Figure 5.

Expression of STAMBP/STAMBP was directly regulated by miR-99a-3p in HNSCC cells. (A) Expression of STAMBP mRNA was significantly reduced by miR-99a-3p transfection into FaDu and SAS cells (72 h after transfection; * p < 0.0001, N.S.: Not significant). Expression of GAPDH was used as an internal control. (B) Expression of STAMBP protein was reduced by miR-99a-3p transfection into HNSCC cells (72 h after transfection). Expression of GAPDH was used as an internal control. (C) TargetScanHuman database analyses predicted one putative miR-99a-3p binding site in the 3′-UTR of STAMBP. (D) Dual luciferase reporter assays showed that luminescence activities were reduced by cotransfection with wild-type (miR-99a-3p binding site) vectors and miR-99a-3p in FaDu and SAS cells. Normalized data were calculated as Renilla/firefly luciferase activity ratios (N.S.: Not significant).