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. 2020 Jan 9;11:21. doi: 10.1186/s13287-019-1539-8

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Characteristics of hUCMSCs and hUCMSC-EVs. Morphology of a passage 0 and b passage 3 hUCMSCs were observed under light microscopy (× 100). Representative images of osteocyte (× 100) and chondrocyte (× 200) differentiation of hUCMSCs cultured in the differentiation medium. The cells were stained with c Alizarin Red and d Alcian Blue. e Results for the flow cytometry analyses of phenotypic markers related to MSCs. f hUCMSC-EVs were observed under a transmission electron microscope; some of the hUCMSC-EVs are indicated by arrows. Scale bar = 200 nm. g Size distributions of hUCMSC-EVs were detected using the NTA. h Western blotting analysis of CD63 and Calreticlin expression in lysates from hUCMSC-EVs and hUCMSCs. i Flow cytometry detection of CD73 and CD90. EVs reacted with the isotype antibody were applied as the negative control (NC)