Fig. 3.

Stress induces RAGE upregulation while HMGB1 silencing in the RVLM reduced RAGE protein expression. a TLR2, TRL4, TLR9, and RAGE mRNA expression levels in microglia ex vivo were determined by quantitative real-time PCR. b, c The protein was obtained and used to measure the differences in the TLR2, TRL4, TLR9, and RAGE expressions. d Double immunohistochemical to check RAGE and microglia colocalization in the RVLM from siHMGB1, control, and stressed mice. (scale bar = 50 μm). e Densitometric measurement of RAGE immunopositivities. f The levels of Co-localization of RAGE and OX42 was assessed by using the Pearson coefficient. Data are presented as mean ± SEM. n = 6, *P < 0.05, ANOVA LSD test