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. 2020 Jan 9;18:7. doi: 10.1186/s12964-019-0502-8

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — FOXE1 is a transcription factor of HK2. a Luciferase activity of the pGL3-HK2-Luc in HCT116 and LoVo cells being transfected with FOXE1 plasmid. b The truncated HK2 promoter regions were cloned into the pGL3 plasmid. c Enhanced FOXE1 expression strongly enhanced the promoter activity of the P1 but not the P2, P3 and P4 regions. d ChIP assays performed in HCT116 and LoVo cells. Specific anti-Flag antibody for ectopically expressed Flag-FOXE1, but not isotype IgG, captured the fragment that possibly containing the FOXE1 response element in the HK2 promoter region. *P < 0.05