KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-HA-Peroxidase	Roche	Cat # 12013819001; RRID:AB_439705
Anti-FLAG-Peroxidase	Sigma-Aldrich	Cat # A8592; RRID:AB_259529
Anti-GFP	Roche	Cat # 11814460001; RRID:AB_390913
Anti-Mouse IgG HRP-linked antibody	Cell Signaling	Cat # 7076; RRID:AB_330924
Phospho-p44/42 MAPK (Erk1/2) Antibody	Cell Signaling	Cat #9101; RRID:AB_331646
Anti-rabbit IgG HRP-linked antibody	Cell Signaling	Cat #7074; RRID:AB_2099233
Anti-FLAG M2 Affinity gel	Sigma-Aldrich	Cat # 2220; RRID:AB_10063035
Protein G Agarose	Roche	Cat # 05015952001
Bacterial and Virus Strains
Agrobacterium tumefaciens GV3101	[10]	N/A
E. coli BL21	[42]	N/A
Pseudomonas syringae pv. tomato DC3000 (Pst)	[41]	N/A
P. syringae pv. maculicola ES4326 (Psm)	[44]	N/A
Pst avrRpt2	[42]	N/A
Pst avrRpm1	[43]	N/A
Pst avrRps4	[43]	N/A
Chemicals, Peptides, and Recombinant Proteins
flg22	[48]	N/A
Pep1	[48]	N/A
MG132	AG Scientific	Cat #99533-80-9
K252a	AG Scientific	Cat #133407-82-6
RiboZol™ RNA Extraction Reagent	AMRESCO	Cat # N580
IPTG	Sigma-Aldrich	Cat# I6758
Pierce glutathione agarose	Thermo Scientific	Cat# 16101
amylose resin	New England BioLabs	Cat# E8021L
Luminol	Sigma-Aldrich	Cat#A8511
Peroxidase from horseradish	Sigma-Aldrich	Cat#P6782
RNase-free DNase I	New England BioLabs	Cat # M0303L
Critical Commercial Assays
M-MuLV Reverse Transcriptase	New England BioLabs	Cat # M0253L
iTaq SYBR green Supermix	Bio-Rad	Cat # 1725124
mMESSAGE mMACHINE T7 high yield RNA Transcription Kit	Ambion	Cat # AM1344
Phusion U Hot Start DNA polymerase	Thermo Fisher	Cat # F555
USER enzyme mix	New England Biolabs	M550
Experimental Models: Organisms/Strains
Arabidopsis thaliana Col-0 wild-type	[10]	N/A
bak1–4	[10]	N/A
btl1 (cngc20–3)	ABRC	SALK _013823C
cngc1	ABRC	SAIL_443_B11
cngc2	[16]	dnd1–1
cngc3	ABRC	SALK_056832C
cngc4	[17]	dnd2–1
cngc5	ABRC	SALK_149893C
cngc6	ABRC	SALK_042207
cngc7	ABRC	SALK_060871C
cngc8	ABRC	GABI_101C03
cngc9	ABRC	SALK_026086
cngc10	ABRC	SALK_015952C
cngc11	ABRC	SALK_026568C
cngc12	ABRC	SALK_092657
cngc13	ABRC	SALK_060826
cngc14	ABRC	WiscDsLox437E09
cngc15	ABRC	CS93507
cngc16	ABRC	SAIL_726_B04
cngc17	ABRC	SALK_041923
cngc19	ABRC	SALK_007105
cngc20–1	ABRC	SALK_129133C
cngc20–2	ABRC	SALK_074919C
bak1–4	[10]	N/A
bak1–4/serk4–1	[10]	N/A
pepr1/2	[19]	N/A
bak1–4/cngc20–1	This paper	N/A
bak1–4/serk4–1/cngc20–3	This paper	N/A
bak1–4/serk4–1/cngc20–1	This paper	N/A
bak1–4/serk4–1/cngc20–1/cngc19	This paper	N/A
pCNGC20::gCNGC20-HA/cngc20–3	This paper	N/A
pCNGC20::gCNGC20T560/S617/S618/T619D-HA/cngc20–1	This paper	N/A
p35S::CNGC20-HA/Col-0	This paper	N/A
p35S::CNGC20-HA/bak1–4	This paper	N/A
p35S::AT1G60995-HA/btl1	This paper	N/A
pAT1G60995::AT1G60995-HA/btl1	This paper	N/A
Xenopus laevis	[49]	N/A
Saccharomyces cerevisiae strain W303–1A	H Iida laboratory (Tokyo Gakugei University)	N/A
Saccharomyces cerevisiae strain K927	H Iida (Tokyo Gakugei University).	N/A
Saccharomyces cerevisiae strain AH109	[41]	N/A
Oligonucleotides
Primers for VIGS, cloning and point mutation, see Table S4	This paper	N/A
Primers for genotyping, see Table S4	This paper	N/A
Primers for qRT-PCR and RT-PCR, see Table S4	This paper	N/A
Recombinant DNA
pYL156 (pTRV-RNA2)	[10]	N/A
pTRV-RNA1	[10]	N/A
pYL156-GFP	[10]	N/A
pYL156-BAK1/SERK4	[10]	N/A
pYL156-MEKK1	[10]	N/A
pYL156-BIR1	[10]	N/A
pHBT	[41]	N/A
pGST	[45]	N/A
pMAL-c2	[45]	N/A
pCB302	[10]	N/A
pGADT7	[41]	N/A
pGBKT7	[41]	N/A
pNB1	[49]	N/A
pHEE401E	[47]	N/A
pCBC-DT1T2	[47]	N/A
pHBT-p35S::GCaMP3	This paper	N/A
pYES2	[34]	N/A
pHBT-BAK1-HA	[45]	N/A
pHBT-BIK1-HA	[45]	N/A
pHBT-BAK1-FLAG	[45]	N/A
pHBT-SERK4-FLAG	[45]	N/A
pMAL-BAK1CD-HA	[45]	N/A
pMAL-BAK1CDKM-HA	[45]	N/A
pHBT-CNGC20-HA	This paper	N/A
pHBT-CNGC20-FLAG	This paper	N/A
pHBT-CNGC20-cYFP	This paper	N/A
pHBT-CNGC19-HA	This paper	N/A
pHBT-CNGC19-nYFP	This paper	N/A
pHBT-CNGC20C-HA	This paper	N/A
pHBT-CNGC20N-FLAG	This paper	N/A
pHBT-CNGC20NT145/S146/S183/S184A-HA	This paper	N/A
pHBT-CNGC20CT560A-HA	This paper	N/A
pHBT-CNGC20CT560/S617/S618/T619A-HA	This paper	N/A
pHBT-CNGC20CT560/S617/S618/T619D-HA	This paper	N/A
pHBT-p35S::gCNGC20-HA	This paper	N/A
pHBT-pCNGC20::gCNGC20-HA	This paper	N/A
pGST-CNGC20N	This paper	N/A
pGST-CNGC20C	This paper	N/A
pGST-CNGC20NT145/S146/S183/S184A	This paper	N/A
pGST-CNGC20CT560A	This paper	N/A
pGST-CNGC20CT560/S617/S618/T619A	This paper	N/A
pGADT7-BAK1K	This paper	N/A
pGBKT7-CNGC20N	This paper	N/A
pCAMBIA1300-pCNGC20::gCNGC20-HA	This paper	N/A
pCAMBIA1300-pCNGC20::gCNGC20T560/S617/S618/T619D-HA	This paper	N/A
pNB1YFP-CNGC19	This paper	N/A
pNB1YFP-CNGC20	This paper	N/A
pNB1YC-CNGC19	This paper	N/A
pNB1YN-CNGC20	This paper	N/A
pHEE401E-CNGC19	This paper	N/A
pCB302–35S::CNGC20-HA	This paper	N/A
pCB302-p35S::AT1G60995-HA	This paper	N/A
pCB302-pAT1G60995::AT1G60995-HA	This paper	N/A
pCB302-pBAK1::BAK1-FLAG	This paper	N/A
pYES2-CNGC19	This paper	N/A
pYES2-CNGC20	This paper	N/A
Software and Algorithms
Bowtie aligner software	[50]	http://bowtie-bio.sf.net
CLC Genomics Workbench 6.0.1 software	QIAGEN	http://www.clcbio.com
LTQ Orbitrap XL LC-MS/MS system	Thermo Scientific	N/A
Mascot	Matrix Science	Version 2.2.2
Olympus Fluoview Viewer	Olympus	Version 3.0
ZEN	Zeiss	https://www.zeiss.com/microscopy/int/products/microscope-software/zen-lite.html
LAS-X	Leica	https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/
ImageJ	NIH	https://imagej.nih.gov/ij/