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. 2019 Jul 10;18(2):337–353. doi: 10.1111/pbi.13201

Figure 2.

Figure 2

Phenotypes of MdMYB308L transgenic apple calli and Arabidopsis seedlings under cold stress. (a) Appearance of 8‐day‐old apple calli under control (24°C) and cold stress (4°C) conditions for 10 days. WT: wild‐type; MdMYB308L‐OX: MdMYB308L‐overexpression; MdMYB308L‐Anti: MdMYB308L antisense suppression. (b) The fresh weight of apple calli shown in (a). (c) Expressions of MdCBFs and their target genes in apple calli shown in (a). Quantitative real‐time PCR analysis was performed in three biological replicates and three technical replicates. The value for WT was set to 1. (d) Appearance of control (Col‐0) and MdMYB308L overexpression (MdMYB308L‐L1, MdMYB308L‐L2 and MdMYB308L‐L3) Arabidopsis seedlings with or without (control) cold stress treatment. Plants were grown on MS plates at 22°C for 12 days before cold treatment at ‐4°C for 0.5 h. (e) Survival rate of Arabidopsis seedlings shown in (d). Each experiment was performed in three replicates. Error bars denote standard deviation. Different letters above the bars indicated significant difference (P < 0.05) as obtained by one‐way ANOVA and LSD test.