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. 2019 Jul 10;18(2):337–353. doi: 10.1111/pbi.13201

Figure 9.

Figure 9

Phenotypes of MdMIEL1 transgenic apple calli, Arabidopsis seedlings and apple leaves. (a) Appearance of 8‐day‐old apple calli under control (24°C) and cold stress (4°C) conditions for 10 days. WT: wild‐type; MdMIEL1‐OX: MdMIEL1‐overexpression; MdMIEL1‐Anti: MdMIEL1 antisense suppression. (b) The fresh weight of apple calli shown in (a). (c) The appearance of control (Col‐0) and MdMIEL1 overexpression (MdMIEL1‐L1, MdMIEL1‐L2 and MdMIEL1‐L3) Arabidopsis seedlings with or without (control) cold stress treatment. Plants were grown on MS plates at 22°C for 12 days before cold treatment at −4°C for 10 min. (d) Survival rate of Arabidopsis seedlings shown in (c). (e) Appearance of apple calli (15‐day‐old) used in (a) when treated with highlight for 7 days. WT: wild‐type; MdMIEL1‐OX: MdMIEL1‐overexpression; MdMIEL1‐Anti: MdMIEL1 antisense suppression. (f) The relative anthocyanin content of apple calli shown in (e). The value for WT was set to 1. (g) Appearance of apple leaves expressing different constructs after highlight treatment for 5 days. EV: empty vector; MdMIEL1‐OX: MdMIEL1‐overexpression; MdMIEL1‐Anti: MdMIEL1 antisense suppression. (h) The relative anthocyanin content of apple leaves shown in (g). The value for EV was set to 1. Each experiment was performed in three replicates. Error bars denoted standard deviation. Significant differences were detected by t‐test (**P < 0.01). Different letters above the bars indicated significant difference (P < 0.05) as obtained by one‐way ANOVA and LSD test.