Skip to main content
. 2019 Jul 10;18(2):337–353. doi: 10.1111/pbi.13201

Figure 11.

Figure 11

MdMIEL1 decreases MdMYB308L‐promoted cold tolerance and anthocyanin accumulation. (a) Appearance of apple calli of 8‐day‐olds expressing different constructs under control condition (24°C) or cold treatment condition (4°C) for 10 days. WT: wild‐type; MdMYB308L‐OX: MdMYB308L‐overexpression; MdMYB308L‐OX/MdMIEL1‐OX: overexpression of MdMIEL1 in the background of MdMYB308L‐overexpression; MdMYB308L‐OX/MdMIEL1‐Anti: suppression of MdMIEL1 in the background of MdMYB308L‐overexpression. (b) The fresh weight of apple calli shown in (a). (c) Appearance of wild‐type (Col‐0) and transgenic Arabidopsis seedlings under control condition or cold treatment condition. Arabidopsis seedlings were grown on MS plates at 22°C for 12 days before being treated at −4°C for 0.5 h. MdMYB308L‐L1 and MdMYB308L‐L2: MdMYB308L‐overexpression Arabidopsis seedlings. MdMYB308L‐L1/MdMIEL1‐L1 and MdMYB308L‐L2/MdMIEL1‐L1: MdMIEL1 and MdMYB308L co‐transformed Arabidopsis seedlings. Control: no treatment; cold treatment: grow at 4°C for 2 days, followed by cold treatment at −4°C for 0.5 h. (d) Survival rate of Arabidopsis seedlings after cold stress treatment. (e) Appearance of 15‐day‐old apple calli treated with highlight for 5 days. (f) The relative anthocyanin content of apple calli shown in (e). The value for WT was set to 1. (g) Appearance of apple leaves treated with highlight for 5 days. EV: empty vector; MdMYB308L‐OX: MdMYB308L‐overexpression; MdMYB308L‐OX/MdMIEL1‐OX: overexpression of MdMIEL1 in the background of MdMYB308L‐overexpression; MdMYB308L‐OX/MdMIEL1‐Anti: suppression of MdMIEL1 in the background of MdMYB308L‐overexpression. (h) The relative anthocyanin content of apple leaves shown in (g). The value for EV was set to 1. Each experiment was performed in three replicates. Error bars denoted standard deviation. Different letters above the bars indicated significant difference (P < 0.05) as obtained by one‐way ANOVA and LSD test.