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. 2019 Jul 24;18(2):354–363. doi: 10.1111/pbi.13202

Figure 6.

Figure 6

SlMIXTA‐like directly binds to the promoter of SlDAHPS to induce its expression. (a) Yeast one‐hybrid indicates SlMIXTA‐like directly interacts with the MRE in SlDAHPS promoter. The SlDAHPS promoter sequence from −113 to −81 containing the MRE motif was repeated three times and fused to the HIS2 reporter gene in Y1H assay. AtMYB12 was used a positive control. (b) SlMIXTA‐like directly binds to MRE in proSlDAHPS to induce its expression. The SlDAHPS promoter sequence (−1000 to + 106) containing the MRE motif (from −99 to −91) was inserted into the reporting system. A mutated version (∆ MRE) was designed as a negative control. Error bars show SEM (n = 3). Different letters indicate significantly different values at P < 0.05 (one‐way ANOVA, Tukey's post hoc test).