Fig. 4.

H19 up-regulation elevates cell proliferation and differentiation of BMSCs through mediating miR-19b-3p. (a) The expression of H19 in the serum of postmenopausal osteoporosis patients and heathy controls were measured by qRT-PCR. Each specimen was repeated three times. (b) Control group, normal BMSC cell; BMP-2 group, BMSC cell treated with 100 ng/mL BMP-2. *P < 0.05 versus healthy control group. Control group, BMSC cells treated with BMP-2; H19 group, BMP-2 treated cells transfected with pcDNA3.1-H19; pcDNA3.1 group, BMP-2 treated cells transfected with pcDNA3.1 empty vector; H19 + miR-19b-3p mimic, BMP-2 treated cells co-transfected with pcDNA3.1-H19 and miR-19b-3p mimic. H19 (c) and miR-19b-3p (d) expression were measured by qRT-PCR. (e) Cell proliferation was measured by BrdU assay. (f) ALP activity was detected in the supernatant of cells. (g) Protein expression of RUNX2 and COL1A1 were measured by western blot method. (h and i) Relative protein level was normalized to GAPDH. *P < 0.05 versus healthy control group. *P < 0.05 versus control group; #P < 0.05 versus H19 group