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. 2020 Jan 9;20:3. doi: 10.1186/s12896-020-0598-3

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Urea inhibits intein self-cleavage of CI-DPIP. a: SDS-PAGE analysis of cleavage in the buffer with different concentrations of urea. M: Molecular weight markers. Lane 1: input. Lanes 2 and 3: resin after cleavage by 2 M and 1 M urea-containing buffers. 4: resin after cleavage without urea. b: calculated cleavage ratio of every cleavage reaction. All the cleaving reaction was completed in the TGE buffer (pH 5.5) at 25 °C for 2 days