Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 9;20:3. doi: 10.1186/s12896-020-0598-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 6 — Purification and mass spectroscopy confirm B22D desb30 insulin. a: HPLC of B22D desB30 insulin. The UV absorption was detected at 280 nm. Conditions: equipment, HPLC Waters 600 (Waters, USA). Column: Kromasil C8 column (4.6 × 250 mm). Mobile phase: buffer G (0.1% TFA), buffer H (90% HPLC grade acetonitrile in H2O, 0.08% TFA). Gradient: 35–50% buffer B in 30 min. Flow rate: 1 ml/min. Injected volume: 200 μl. b: mass spectroscopy analysis of B22D desB30 insulin. The determined MW is 5664.0 Da