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. 2020 Jan 9;20:3. doi: 10.1186/s12896-020-0598-3

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — SEC elution profile of B22D desb30 insulin compared with commercial sample. Size exclusion chromatography of a): commercially available insulin sample and b). B22D desB30 insulin. The UV absorption was detected at 280 nm. Column: GE Superdex 75 Flow rate: 0.5 ml/min. FPLC: GE Healthcare Purifier 100