Table 1.

Summary of DPIP Purification

c	Total Protiena(mg)	Target proteinb(mg)	Approximate purityc(%)	Yieldd(%)
Cell lysis	200.33	133.82 (CBD-intein-DPIP)	66.8	100
Refolding of fusion protein	156.26	116.73 (CBD-intein-DPIP)	74.7	87.2
Chitin column and intein-mediated cleavage	0.621	0.548 (DPIP)	88.3	2.4

One liter of culture contained about 3.2 g wet weight cells

^a: Total protein was quantified by Bradford assay

^b: The amount of fusion protein was calculated based on the following formula: target protein = total protein amount x approximate purity

^c: The purities of fusion protein were determined by densitometry analysis of their corresponding SDS gel bands

^d: The relative low recovery of DPIP was partially due to the low molecular weight of DPIP (6 kDa) compared to the fusion precursor CBD-intein-DPIP (33 kDa)