Table 2.

Buffers used in protein purification

Buffers	Details
Buffer A	50 mM Tris-HCl, 2 mM EDTA, 100 mM NaCl
Buffer B	50 mM Tris-HCl, 1 mM EDTA, 100 mM NaCl,1% Tween
Buffer C	50 mM Tris-HCl, 1 mM EDTA, 100 mM NaCl, 1% Tween, 4 M Urea
Buffer D	50 mM Tris-HCl, 1 mM EDTA, 100 mM NaCl, 3% Tween
Buffer E	50 mM Tris-HCl, 1 mM EDTA, 100 mM NaCl,0.5% Tween
Buffer F	50 mM Tris-HCl, 1 mM EDTA, 10 mM β- Mercaptoethanol, 2 mM Sodium deoxycholate pH 8.0, 8 M Urea
TGE buffer	50 mM Tris-HCl, 0.5 mM EDTA,50 mM NaCl, 5% Glycerol, 1% Glycine, 0.1% Glutathione pH 8.0
Digestion buffer	20 mM Tris–HCl, 200 mM NaCl and 1 mM EDTA, pH 8.0
Buffer G	0.1% TFA
Buffer H	90% HPLC grade acetonitrile in H2O, 0.08% TFA