Fig. 2.

TEAD4 activates MNX1-AS1 expression in GC cells. a JASPAR database was used to predict TEAD4 binding site on the promoter region of MNX1-AS1. b TEAD4 expression in GC tissues (n = 300) and surrounding tissues (n = 100) in the GSE62254 dataset. c The relationship between MNX1-AS1 and TEAD4 was determined by analyzing GSE62254 data. d The expression level of TEAD4 in GC cells were determined in GC cells transfected with TEAD4 siRNAs or pcDNA-TEAD4 using qRT-PCR assay. e The protein level of TEAD4 was detected in GC cells transfected with TEAD4 siRNAs or pcDNA-TEAD4. f MNX1-AS1 expression was determined in GC cells transfected with TEAD4 siRNAs or pcDNA-TEAD4 using qRT-PCR assay. g ChIP assays showed endogenous TEAD4 binding to the MNX1-AS1 gene promoter. h ChIP assays demonstrated TEAD4 enrichment on the promoter region of MNX1-AS1 with transfection with si-TEAD4. i ChIP assays demonstrated TEAD4 enrichment on the promoter region of MNX1-AS1 with transfection with pcDNA-TEAD4. j Dual luciferase reporter assays revealed that TEAD4 binds to the E2 promoter region (− 1500 to − 1700) of MNX1-AS1. k Dual luciferase reporter assays were performed to determine the exact TEAD4 binding site on the E2 region (− 1500 to − 1700) of MNX1-AS1. * p < 0.05, ** p < 0.01