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. 2020 Jan 10;19:6. doi: 10.1186/s12943-019-1104-1

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© The Author(s). 2020

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Regulation relationship between MNX1-AS1 and miR-6785-5p. a Prediction of potential miRNAs targeting lncRNA MNX1-AS1 were analyzed using miRanda, pita, and RNAhybrid databases. b-d The luciferase reporter plasmid (pMIR-GLO-MNX1-AS1) was cotransfected into HEK-293 T cells with 5 miRNA-coding plasmids. The interacting activity between miRNAs and MNX1-AS1 was clarified using luciferase reporter assays. e, f MNX1-AS1 knockdown could obviously upregulate miR-6785-5p in GC cells, and MNX1-AS1 overexpression could significantly downregulate miR-6785-5p. g No significant difference in MNX1-AS1 expression after overexpression of miR-6785-5p in GC cells. h RNA levels in immunoprecipitates are presented as fold enrichment in AGO2 relative to IgG immunoprecipitates in SGC7901 and MGC803 cells