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. 2020 Jan 9;20:8. doi: 10.1186/s12866-020-1695-0

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Enumeration of Fno in Galleria mellonella larva after injection. Abundance of Fno STIR-GUS-F2f7 (black line) and Fno Ehime-1 (grey line) in the haemolymph of G. mellonella larvae during 264 h at 28 °C after injection with ca. 1 × 10⁹ CFU/mL. Actual CFU/mL after plating bacterial suspensions of each Fno isolate on CHAH were: 5.7 × 10⁹ for Fno STIR-GUS-F2f7 and 1.6 × 10⁹ for Fno Ehime-1. Unmanipulated and PBS only larvae were sampled at the start, middle (144 h) and end of the experiment and no Fno colonies were recovered (data not shown). Bars are means of log₁₀ transformations of (CFU/mL + 1) data + one standard deviation (n = 5)