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. Author manuscript; available in PMC: 2020 Jan 10.
Published in final edited form as: Cell Rep. 2019 Dec 24;29(13):4460–4470.e8. doi: 10.1016/j.celrep.2019.11.084

Figure 5. mAb 019-10117 3C06 Requires RBS Contacts and Bivalent Binding for Cross-Reactivity.

Figure 5.

(A and B) ELISAs were completed using the 019-10117-3C06 mAb (A) and the 024-10128-3C04 mAb (B) and plates coated with mammalian-expressed recombinant A/Victoria/210/2009 (Vic/09) HA VLPs with only Y98F, only K160T, or Y98F and K160T. ELISA binding curves from experimental triplicates are shown with one site—specific binding curves fit to the data (GraphPad Prism). (C–F) Dashed lines represent the 95% confidence interval (CI) for each curve fit. Biolayer interferometry (BLI) was used to measure binding of 019-10117-3C06 IgG and Fab to A/Perth/16/2009 (Perth/09) (C and D) and A/Hong Kong/1/1968 (HK/68) (E and F) baculovirus-expressed recombinant HA proteins. Gray lines represent the response curve and colored lines represent the 1:1 binding model (for Fab) or heterogenous ligand model (for IgG).