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. Author manuscript; available in PMC: 2020 Jan 10.
Published in final edited form as: Matrix Biol. 2015 Dec 12;49:61–81. doi: 10.1016/j.matbio.2015.12.005

Fig. 1.

Fig. 1.

Biglycan-induced IL-1β expression and maturation in macrophages is regulated by NAPDH oxidase-derived ROS. (A) ELISA for mature IL-1β in the cell culture media from wild-type macrophages pre-treated for 1 h with DPI (0.5 μM) or VAS2870 (5 μM), followed by 16 h biglycan (4 μg/ml) stimulation; n = 5. (B) Western blot analysis of pro- and mature IL-1β in macrophages after 1 h pre-incubation with VAS2870 and stimulation with biglycan for 6 h for pro-IL-1β and 16 h for mature IL-1β. β-actin served as loading control. (C) Quantification of pro-IL-1β normalized to β-actin and given as fold induction to untreated control from 3 independent experiments. (D) Quantification of mature IL-1β normalized to β-actin and given as fold induction to untreated control from 3 independent experiments. (E) Quantitative RT-PCR for II1β mRNA expression in primary murine macrophages after 6 h stimulation with soluble biglycan and 1 h pre-incubation with VAS2870, normalized to Gapdh and given as fold induction over untreated controls; n = 5. (A, C–E) Data are given as means ± S.D.; *P < 0.05.