Biglycan-mediated NOX2 activation is dependent on Erk and Rac1. (A) Confocal images of F4/80 (green) and p47phox (red) in WT macrophages with or without biglycan treatment for 40 min, after 1 h treatment with Rac1 inhibitor (50 μM), MEK1/2 inhibitor (10 μM), p38 MAPK inhibitor (10 μM), or PKC inhibitor (20 nM). Inserts in the confocal images represent line-scanned profiles, while the white arrows indicate the distance within which the scans were generated. Bar = 10 μm. (B) Quantification of p47phox co-localization with F4/80 from (A) given as dot plot for the mean correlation index (Icorr) ± S.D. of at least 10 cells per condition; *P < 0.05.