KEY RESOURCES TABLE
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| CD34 PE-Cy7 Mouse anti-human | BD Pharmingen | 560710 |
| CD34 PE Mouse anti-human | BD Pharmingen | 555822 |
| CD38 APC Mouse anti-human | BD Pharmingen | 555462 |
| CD43 BV711 Mouse anti-human | BD OptiBuild | 743614 |
| CD43 FITC Mouse anti-human | BD Pharmingen | 555475 |
| CD45 V450 Mouse anti-human | BD Horizon | 560367 |
| CD45RA APC—H7 Mouse anti-human | BD Pharmingen | 560674 |
| CD49f PE-Cy5 Rat anti-human | BD Pharmingen | 551129 |
| CD73 PE Mouse anti-human | BD Pharmingen | 550257 |
| CD73 FITC Mouse anti-human | BD Pharmingen | 561254 |
| CD90 PE-Cy7 Mouse anti-human | BD Pharmingen | 561558 |
| CD105 AF647 Mouse anti-human | BD Pharmingen | 561439 |
| CD144 BV605 Mouse anti-human | BD OptiBuild | 743705 |
| CD184 PE-CF594 Mouse anti-human | BD Horizon | 562389 |
| CD235a FITC Mouse anti-human | Biolegend | 349104 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant | Proteins | |
| ROCK Inhibitor | Sigma | Y0503 |
| CHIR99021 | Sigma | SML1046 |
| SB431542 | Sigma | S4317 |
| Erythropoietin (EPOGEN®) | Amgen | NDC 55513-126-10 |
| Interleukin-3 (IL3) | R&D Systems | 203-IL |
| Stem Cell Factor (SCF) | R&D Systems | 255-SC |
| Estradiol | Pfizer | NDC 0009-0271-01 |
| Dexamethasone | VETone | NDC 13985-533-03 |
| Knockout Replacement Serum | ThermoFisher | 10828028 |
| Transferrin | Sigma Aldrich | T4132–1 G |
| Bovine Serum Albumin | Roche | 03116956001 |
| Insulin | Lilly | Humulin R HI210 |
| Critical Commercial Assays | ||
| RNEasy Micro Kit | Qiagen | 74004 |
| Taqman RNA-to-Ct 1-Step Kit | Applied Biosystems | 4392653 |
| MethoCult H4435 Enriched Human CFU Assay | STEMCELL Technologies, Inc. | 04445 |
| Adipocyte Differentiation Assay | ThermoFisher | A1007001 |
| Chondrocyte Differentiation Assay | ThermoFisher | A1007101 |
| Osteocyte Differentiation Assay | ThermoFisher | A1007201 |
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| MCND-TENS2 Line | This Paper | RTIBDi001-A |
| OP9 | ATCC | CRL-2749 |
| Experimental Models: Organisms/Strains | ||
| Female NSG Mice | Jackson Laboratory | 05557 |
| Oligonucleotides | ||
| GAPDH – VIC | Life Technologies | Hs03929097_g1 |
| HoxA5 – FAM | Life Technologies | Hs00430330_m1 |
| HoxA9 – FAM | Life Technologies | Hs00365956_m1 |
| HoxA10 – FAM Recombinant DNA | Life Technologies | Hs00172012_m1 |
| Software and Algorithms | ||
| FlowJo 10.5.3 | FlowJo, LLC | N/A |
| GraphPad Prism 7.02 | GraphPad Software, Inc. | |
| Other | ||
| Matrigel | Corning | 354230 |
| Essential 8 Medium | ThermoFisher | A1517001 |
| STEMdiffTM Hematopoietic Kit | STEMCELL Technologies, Inc. | 05310 |
| 7-AAD | Thermofisher | 00-6993-50 |
| Accutase | STEMCELL Technologies, Inc. | 07920 |