Table 1.

454 antibody sequencing reveals high sequence diversity of eAd libraries generated by engineering of CDRs or loops.

eAd libraries	Numbers of unique eAds	Numbers of unique CDRs
		CDR1	CDR2	CDR3
m81a	7,770	956	2,398	6,731
m91b	26,197	11,518	6,140	21,553
m01slc	37,818	28,626d	2,463e	15,645f

^aA human VH library based on m0 scaffold and grafting of in vivo-formed HCDR2s and HCDR3s from our other human antibody libraries onto their respective CDRs, and mutating four residues in the CDR1 of m0 [15].

^bA second-generation human VH library constructed by grafting of LCDR3s from our other human antibody libraries into the CDR1 of library m8l [45].

^cA human IgG1 CH2 domain-based eAd library using m01s as a framework scaffold in which the FG loop was replaced by the HCDR3s from library m8l, and mutations were intro duced to BC and DE loops by site-directed mutagenesis [13].

^dBC loop;

^eDE loop;

^fFG loop