Figure 3.
Multi-scale Simulations Predict In Vivo Responses of L5PTs
(A) Numerical simulations of current injections into the soma and/or Ca2+ channel dense region of the apical dendrite of a biophysically detailed multi-compartmental model of the L5PT shown in Figure 2.
(B) The model supports regular AP firing of increasing frequencies in response to sustained current injections of increasing amplitude.
(C) The peak conductance of synapses was optimized to match empirically determined unitary postsynaptic potential (uPSP) amplitude distributions (here: VPM-to-L5PT synapses).
(D) From left to right panels: membrane potential along the dendrites of the model for exemplary simulation of PW deflection. Somatic AP for exemplary simulations of PW and SW stimulation is shown. Raster plots of APs for 200 randomly selected simulation trials reflecting different plausible synaptic input patterns. Exemplary wRFs for three different L5PT models are shown.
(E) Post-stimulus time histograms (PSTHs) of APs during stimulation of the PW and the eight SWs. Solid lines and shaded areas represent the means and SDs across in vivo recorded L5PTs (n = 9) and L5PT models (n = 9), respectively.
(F) In silico versus in vivo average wRF of L5PTs. Error bars denote SEMs.
(G) Distribution of the correlations between in vivo and in silico wRFs (red) versus correlations with a null distribution (black) of synthetically generated random wRFs.