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. 2019 Dec 31;25:10198–10204. doi: 10.12659/MSM.919690

Figure 3.

Figure 3

MiR-34a overexpression attenuates the stemness-like traits of A549 cells via targeting IL-6R. (A) The activity of Luc-IL-6R-wt and Luc-IL-6R-mut was evaluated in A549 cells with or without miR-34a overexpression. (B) IL-6R expression was measured in A549 cells with or without miR-34a overexpression. (C) Sphere size was measured in A549 cells with miR-34a overexpression as well as IL-6 treatment or IL-6R overexpression or not. (D) Sphere number was determined in A549 cells with miR-34a overexpression plus IL-6 treatment or IL-6R overexpression or not. (E, F) RT-qPCR and western blot analysis on the A549 cells described in (C). (G) ALDH1 activity was examined in A549 cells with miR-34a overexpression as well as IL-6 treatment or IL-6R overexpression or not. ** P<0.01 versus control. MiR-34a – microRNA-34a; IL-6 – interleukin-6; MCT-1 – multiple copies in T-cell malignancy 1; RT-qPCR – real-time quantitative polymerase chain reaction; ALDH1 – aldehyde dehydrogenase 1.