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. 2019 Dec 31;25:10198–10204. doi: 10.12659/MSM.919690

Figure 4.

Figure 4

MCT-1 regulates the stemness-like traits of A549 cells through the miR-34a/IL-6/IL-6R axis. (A) RT-qPCR analysis on IL-6R and miR-34a levels in A549 cells with MCT-1 knockdown or not. (B) Western blot analysis on IL-6R level in A549 cells infected with Len-MCT-1-kd or not. (C, D) Spheroid forming ability was evaluated in A549 cells infected with Len-MCT-1-kd plus miR-34a knockdown or IL-6R overexpression. (E, F) CD133 and ALDH1 expression was evaluated in A549 cells with MCT-1 knockdown as well miR-34a knockdown or IL-6R overexpression. (G) ALDH1 activity was examined in A549 cells with MCT-1 knockdown plus miR-34a knockdown or IL-6R overexpression. ** P<0.01 versus control. MCT-1 – multiple copies in T-cell malignancy 1; MiR-34a – microRNA-34a; RT-qPCR – real-time quantitative polymerase chain reaction; IL-6 – interleukin-6; ALDH1 – aldehyde dehydrogenase 1.