TABLE 1.
Summary of kits tested
| Kit | Method | miRNA detection | Reproduci-bility | miRNA discovery | Size selection | Prep time, d | Reduced jackpotting | Low input | UMI correction |
|---|---|---|---|---|---|---|---|---|---|
| Takara | Template switching | X | X | X | Pippin Prep | 1.75 | X | X | |
| Diagenode | Template switching | X | X | X | SPRI | 1 | X | X | |
| Trilink | Sequenital ligation | X | X | X | SPRI | 1.2 | |||
| Qiagen | Sequenital ligation | X | X | X | None | 1.2 | X | ||
| Lexogen | Sequenital ligation | X | X | X | SPRI | 1 | |||
| New England Biolabs | Sequenital ligation | X | X | X | Pippin Prep | 1 | |||
| PerkinElmer | Sequenital ligation | X | X | X | SPRI | 2.5 | X | ||
| Illumina | Sequenital ligation | X | X | X | Pippin Prep | 1.3 | |||
| Somagenics | Circular-ization | X | X | X | None | 1.5 | X | ||
| NanoString | Probe hybrid-ization | X | X | None | 2 | X |
SPRI, solid phase reversible immobilization; UMI, unique molecular identifier.