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. 2020 Jan 7;12:100242. doi: 10.1016/j.bonr.2020.100242

Fig. 5.

Fig. 5

Analysis of mice transplanted with human CD34+ cells. a FACS analysis of the BM of a representative NSG oc/oc mouse 2 weeks after transplantation. Human CD45+ leukocytes derived from transplanted CD34+ cells were gated (left pseudocolor plot) and analyzed for the expression of the human CD14 monocyte marker (histogram on the right, blue line; gray line represents the Fluorescent-Minus-One control). b Scatter plots showing (left) the percentage of human CD45+ leukocytes within the BM and the spleen of NSG and NSG oc/oc mice, and (right) the percentage of human CD14+ cells within the BM. c Representative immunohistochemical staining of vertebral bone of transplanted mice two weeks after transplantation, showing the presence of human CD45+ cells. Scale bar: 200 μm. d MicroCT analysis of the femur of NSG and NSG oc/oc mice (n = 3 per group) and NSG oc/oc mice transplanted with human cells (n = 5).