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. Author manuscript; available in PMC: 2020 Jan 10.
Published in final edited form as: Methods Mol Biol. 2019;1982:429–446. doi: 10.1007/978-1-4939-9424-3_25

Fig. 4.

Fig. 4

Results of the screening of a library of bioactive compounds (~2000) using three probes: HPr+ in the presence of DNA as a probe for O2Superscript>/Superscript> and CBA or Amplex Red in the presence of HRP as probes for H2O2. (a) Correlation of the results of the three assays for NOX2 activity. (b) Results of screening as a percentage of positive hits in one, two, or all three assays. (This research was originally published in Journal of Biological Chemistry. Zielonka, J., Zielonka, M., VerPlank, L., Cheng, G., Hardy, M., Ouari, O., Ayhan, M. M., Podsiadly, R., Sikora, A., Lambeth, J. D., & Kalyanaraman, B. Mitigation of NADPH oxidase 2 activity as a strategy to inhibit peroxynitrite formation. J. Biol. Chem. 2016; 291:7029–7044. © the American Society for Biochemistry and Molecular Biology)