Table 4.

Summary of enzymatic activity using different substrates.

Enzyme	Substrate	Duration	Temp.	Assay	Observation	Reference
Lipases	Tween 20	48 h	28 °C		Halo	Sierra (1957)
Esterase	Glyceryl Tributyrate	48 h	28 °C		Halo	Sierra (1957)
Proteases	Casein	24 h	28 °C		Halo	Vieira (1999)
Cellulases	Cellulose, Carboxymethylcellulose	24 h	28 °C	Congo red	Halo	Stamford et al., 1998
Pectinases	Pectin	24 h	28 °C	Lugol solution	Halo	Andro et al. (1984)
Peeroxidases/Laccases	Lignin	48 h	28 °C		Halo	Bonugli-santos et al., 2015
Amylases	Starch	24 h	28 °C	Lugol solution	Halo	Castro et al. (1993)
Chitinases	4-methylumbelliferylN-acetyl-b-D-glucosaminide solution	30minutes	37 °C	Phospahte buffer solution	Fluorecence under U.V light	(Chand et al., 2008)