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. Author manuscript; available in PMC: 2020 May 18.
Published in final edited form as: Nat Struct Mol Biol. 2019 Nov 18;26(12):1106–1113. doi: 10.1038/s41594-019-0327-6

Extended Data Fig. 4. Mechanistic diagram of atypical T-box translational regulation.

Extended Data Fig. 4

a, tRNA recruitment and decoding. b, Pseudoknot formation positions AntiS to interact with tRNA NCCA sequence. c, Transient intermediate where aminoacylation sensing module interacts with uncharged (top) and charged (bottom) tRNA. d, Favorable interactions between uncharged tRNA and linker locks T-box into ON conformation. Exposed Shine-Dalgarno allows translation initiation. e, Steric clashing between charged tRNA and linker unravels the aminoacylation sensing module leading to alternative base pairing. Sequestrator formation prevents ribosome access to Shine-Dalgarno and prevents translation.