Figure 5. Deletion of Tsc2 enhances mTORC1 signaling and augments myelopoiesis.

A) FACS plots of bone marrow Ly6C^hi monocytes (box) in Tsc2 KO and controls. Histograms depict myeloid marker expression in Ly6C^hi monocytes. B) FACS plots of isolated spleen cells from Tsc2 KO and controls. Boxes indicate Ly6C^hi monocytes (blue) and neutrophils (red). C) Giemsa-Wright staining of isolated spleen cells and quantification of large macrophages (arrow; n = 9 HPF counted per group from 3 animals). D) Quantification of splenic progenitor cell populations in Tsc2 KO and controls (n = 3 per group). E) FACS analysis and F) Giemsa-Wright staining of macrophages differentiated from sorted LSK cells from control or Tsc2 KO mice maintained in SCF-containing medium (10 ng/mL; n = 2 per condition). * p < 0.05, ** p < 0.01, *** p< 0.001