Table 3.
Incorporation of [125I]-BMIPP and [3H]-BROMO into various lipid fractions in tissues at rest (n = 5) and exercise (n = 5) as determined by thin layer chromatography
| Tissue | Lipid fraction | [3H]-BROMO | [125I]-BMIPP |
|---|---|---|---|
| Rest | |||
| Heart | PL + MG + DG | 45.5 ± 7.3# | 49.3 ± 9.6# |
| FFA | 29.9 ± 8.7# | 41.4 ± 10.1 | |
| TG | 32.7 ± 4.1 | 9.3 ± 2.3* | |
| Skeletal muscle | PL + MG + DG | 50.7 ± 10.9 | 45.0 ± 11.7 |
| FFA | 36.7 ± 7.1 | 41.2 ± 13.0 | |
| TG | 15.6 ± 6.1 | 14.0 ± 4.3 | |
| Liver | PL + MG + DG | 59.6 ± 6.3 | 37.9 ± 11.5 |
| FFA | 22.2 ± 6.3 | 49.2 ± 13.3 | |
| TG | 19.2 ± 2.5 | 12.9 ± 2.6 | |
| Exercise | |||
| Heart | PL + MG + DG | 69.7 ± 8.9 | 83.4 ± 9.5 |
| FFA | 13.0 ± 1.7 | 2.4 ± 1.0* | |
| TG | 17.3 ± 7.6 | 11.2 ± 6.6 | |
| Skeletal muscle | PL + MG + DG | 43.7 ± 6.1 | 67.4 ± 7.8 |
| FFA | 34.9 ± 5.5 | 22.6 ± 5.7 | |
| TG | 24.4 ± 5.0 | 6.3 ± 1.5* | |
| Liver | PL + MG + DG | 54.0 ± 8.6 | 49.4 ± 15.4 |
| FFA | 25.0 ± 8.7 | 39.4 ± 14.7 | |
| TG | 21.0 ± 8.8 | 11.2 ± 5.3 |
Values are expressed as a percentage of total radioactivity and represent means ± SEM.
PL phospholipid, MG monoglyceride, DG diglyceride, FFA free fatty acids, TG triglyceride.
*
Represents a significant difference between [125I]-BMIPP and [3H]-BROMO within an experimental protocol
#
Represents a significant difference with [125I]-BMIPP or [3H]-BROMO between rest and exercise protocols