Table 1.
Cryptosporidium species and genotypes based on amplification of the small subunit ribosomal rRNA (SSU), actin, Cryptosporidium oocyst wall protein (COWP) and 60kDa glycoprotein (gp60) genes by PCR in samples of striped field mice (Apodemus agrarius), yellow-necked mice (Apodemus flavicollis) and wood mice (Apodemus sylvaticus) from Austria, Belgium, Bosnia and Herzegovina, Bulgaria, Czech Republic, Finland, France, Germany, Hungary, Latvia, Lithuania, Netherlands, Poland, Romania, Serbia and Slovakia.
| Country | Host | N | Isolate ID | Microscopical positivity (OPG) | Genotyping at the loci | |||
|---|---|---|---|---|---|---|---|---|
| SSU | actin | COWP | gp60 | |||||
| Austria | A. flavicollis | 5 | - | - | - | - | - | - |
| A. sylvaticus | 3 | - | - | - | - | - | - | |
| Belgium | A. flavicollis | 2 | 27677 | Yes (4,000) | C. ditrichi | C. ditrichi | C. ditrichi | - |
| Bosnia and Herzegovina | A. flavicollis | 1 | - | - | - | - | - | - |
| Bulgaria | A. agrarius | 5 | - | - | - | - | - | - |
| A. flavicollis | 3 | - | - | - | - | - | - | |
| A. sylvaticus | 2 | - | - | - | - | - | - | |
| Czech Republic | A. flavicollis | 153 | 14887 | Yes (28,000) | C. ditrichi | C. ditrichi | C. ditrichi | - |
| 14890 | Yes (2,000) | C. ditrichi | C. ditrichi | C. ditrichi | - | |||
| 12690 | Yes (24,000) | C. ditrichi | C. ditrichi | C. ditrichi | - | |||
| 12696 | Yes (52,000) | C. ditrichi | C. ditrichi | C. ditrichi | - | |||
| 5025 | No | C. apodemi | C. apodemi | C. apodemi | - | |||
| 12405 | Yes (4,000) | apodemus genotype I | apodemus genotype I | apodemus genotype I | XVIIa | |||
| 30891 | Yes (2,000) | apodemus genotype I | apodemus genotype I | apodemus genotype I | XVIIa | |||
| 30893 | Yes (6,000) | apodemus genotype I | apodemus genotype I | apodemus genotype I | XVIIa | |||
| 23228 | No | C. tyzzeri | C. tyzzeri | C. tyzzeri | IXaA8 | |||
| 14895 | Yes (2,000) | apodemus genotype I | apodemus genotype I | apodemus genotype I | XVIIa | |||
| 12656 | No | apodemus genotype II | apodemus genotype II | - | XVIIIa | |||
| 21523 | No | C. microti | C. microti | C. microti | - | |||
| 23009 | No | C. microti | C. microti | C. microti | - | |||
| A. sylvaticus | 23 | 12373 | Yes (8,000) | C. ditrichi | C. ditrichi | C. ditrichi | - | |
| Finland | A. flavicollis | 2 | 30329 | Yes (2,000) | C. ditrichi | C. ditrichi | C. ditrichi | - |
| France | A. flavicollis | 16 | 30357 | Yes (6,000) | C. ditrichi | C. ditrichi | - | - |
| 30358 | Yes (2,000) | C. ditrichi | C. ditrichi | - | - | |||
| A. sylvaticus | 4 | 30364 | Yes (4,000) | C. ditrichi | C. ditrichi | - | - | |
| Germany | A. flavicollis | 10 | 12058 | No | C. parvum | C. parvum | C. parvum | IIaA16GlRlb |
| 12062 | No | C. ditrichi | C. ditrichi | C. ditrichi | - | |||
| 12063 | No | C. ditrichi | C. ditrichi | C. ditrichi | - | |||
| Hungary | A. agrarius | 4 | - | - | - | - | - | - |
| Latvia | A. agrarius | 11 | 27716 | Yes (6,000) | apodemus genotype II | apodemus genotype II | - | XVIIIa |
| 27715 | Yes (4,000) | apodemus genotype II | apodemus genotype II | - | XVIIIa | |||
| 27712 | Yes (2,000) | C. ditrichi | C. ditrichi | - | - | |||
| Lithuania | A. agrarius | 3 | 27721 | Yes (2,000) | C. apodemi | C. apodemi | C. apodemi | - |
| Netherlands | A. sylvaticus | 6 | 27675 | Yes (6,000) | C. ditrichi | C. ditrichi | - | - |
| Poland | A. flavicollis | 83 | 12958 | No | C. muris | C. muris | C. muris | |
| 12957 | No | apodemus genotype II | apodemus genotype II | - | XVIIIa | |||
| 151 | No | apodemus genotype I | apodemus genotype I | apodemus genotype I | XVIIa | |||
| 111 | Yes (2,000) | apodemus genotype I | apodemus genotype I | apodemus genotype I | XVIIa | |||
| 436 | Yes (6,000) | C. ditrichi | C. ditrichi | C. ditrichi | - | |||
| 521 | Yes (4,000) | C. ditrichi | C. ditrichi | C. ditrichi | - | |||
| 361 | No | C. apodemi | C. apodemi | C. apodemi | - | |||
| Romania | A. agrarius | 2 | 30374 | Yes (2,000) | C. apodemi | C. apodemi | C. apodemi | - |
| 30375 | No | C. apodemi | C. apodemi | C. apodemi | - | |||
| A. flavicollis | 1 | - | - | - | - | - | ||
| A. sylvaticus | 1 | - | - | - | - | - | ||
| Serbia | A. agrarius | 4 | 30383 | Yes (2,000) | apodemus genotype II | apodemus genotype II | - | XVIIIa |
| A. flavicollis | 14 | 30395 | No | apodemus genotype II | apodemus genotype II | - | XVIIIa | |
| 30390 | Yes (2,000) | apodemus genotype II | apodemus genotype II | - | XVIIIa | |||
| 30387 | Yes (22,000) | C. ditrichi | C. ditrichi | C. ditrichi | - | |||
| 30394 | No | C. ditrichi | C. ditrichi | - | - | |||
| A. sylvaticus | 3 | 30389 | No | C. tyzzeri | C. tyzzeri | C. tyzzeri | IXcA6 | |
| Slovakia | A. agrarius | 33 | 10502 | No | apodemus genotype II | apodemus genotype II | - | XVIIIa |
| 11657 | No | apodemus genotype II | apodemus genotype II | - | XVIIIa | |||
| 10462 | No | C. apodemi | C. apodemi | C. apodemi | - | |||
| 4974 | Yes (2,000) | C. apodemi | C. apodemi | C. apodemi | - | |||
| 4961 | Yes (4,000) | C. apodemi | C. apodemi | C. apodemi | - | |||
| 10479 | No | C. ditrichi | C. ditrichi | C. ditrichi | - | |||
| A. flavicollis | 35 | 11985 | No | C. muris | C. muris | C. muris | ||
| 7799 | No | C. ditrichi | C. ditrichi | C. ditrichi | - | |||
| 4950 | Yes (4,000) | C. ditrichi | C. ditrichi | C. ditrichi | - | |||
| 8131 | No | C. parvum | C. parvum | C. parvum | IIaA16GlRlb | |||
| 10466 | No | C. ditrichi | C. ditrichi | C. ditrichi | ||||
| 30369 | Yes (8,000) | apodemus genotype I | apodemus genotype I | apodemus genotype I | XVIIa | |||
| 8153 | No | apodemus genotype I | apodemus genotype I | apodemus genotype I | XVIIa | |||
| 8049 | No | C. apodemi | C. apodemi | C. apodemi | - | |||
| 8060 | No | C. apodemi | C. apodemi | C. apodemi | - | |||
| 7780 | No | C. apodemi | C. apodemi | C. apodemi | - | |||
| A. sylvaticus | 8 | 30402 | Yes (4,000) | C. apodemi | C. apodemi | C. apodemi | - | |
| 30407 | No | C. apodemi | C. apodemi | C. apodemi | - | |||
Oocysts were quantified by microscopy and reported per gram of faeces (OPG); N – Number of examined samples; ID – identification; No – microscopically negative; Yes – Microscopically positive