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. 2019 Dec 14;19:523–532. doi: 10.1016/j.omtn.2019.12.002

Figure 7.

Figure 7

Effect of MEL on the Expression of H19, miR-675, miR-138, HIF1Α, and eNOS in U251 Cells

(A) Co-transfection of the cells with H19 and miR-138 mimics reduced the luciferase activity of the cells (*p < 0.05, versus control group). (B) Transfection of the cells with wild-type 3′ UTR of eNOS significantly decreased the luciferase activity of miR-138 (*p < 0.05, versus control group). (C) Transfection of the cells with miR-675 mimics significantly decreased the luciferase activity of wild-type 3′ UTR of HIF1α (*p < 0.05, versus control group). (D) MEL dose dependently increased the luciferase activity of H19 promoter (*p < 0.05, versus untreated cells). (E) MEL dose dependently increased the expression of H19 (*p < 0.05, versus untreated cells). (F) MEL dose dependently increased the expression of miR-675 (*p < 0.05, versus untreated cells). (G) MEL dose dependently decreased the expression of miR-138 (*p < 0.05, versus untreated cells). (H) MEL dose dependently downregulated the expression of HIF1α mRNA (*p < 0.05, versus untreated cells). (I) MEL dose dependently upregulated the expression of eNOS mRNA (*p < 0.05, versus untreated cells). (J) MEL dose dependently downregulated the expression of HIF1α protein while upregulating the expression of eNOS protein.