Table 1. Primer sequences used for 16S rRNA and hsp60 genes amplification.
| Gene | Designation | Primers (5′-3′) | Annealing temperature | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
| 16S rRNA | fD1 | AGAGTTTGATCCTGGCTCAG | 52°C | 1475 | [20] |
| rP1 | ACGGTTACCTTGTTACGACTT | ||||
| FrAF | CGATTGAGCCAAGGGCTTTA | 52°C | 461 | This study | |
| Vc1-2R | ACTTAACCCAACATCTCACG | [21] | |||
| FrBF | ACACGTGCTACAATGGCATA | 53°C | 451 | This study | |
| FrBR | TCTCTGAAAACTAAACAAGGATGA | ||||
| hsp60 | hsp60_AF | AACTTTATGGTGGCGTTAAAA | 52°C | 1118 | |
| hsp60_AR | AGTTTCTGTTGCAGCACCTA | ||||
| hsp60_BF | AGCTTAATGTTGTTGAGGGA | 51°C | 1085 | ||
| hsp60_BR | TTACATCATACCACCCATAC |