. 2020 Jan 10;15(1):e0227422. doi: 10.1371/journal.pone.0227422

Table 1. Number of isolates and population densities of diazotrophic/N-scavenging bacteria isolated via different semisolid N-free culture media from soils under different management conditions and from unwashed roots of tomato and lulo plants grown on those soils.

Isolation source^a		Semisolid N-free medium										Total MPN^b
Isolation source^a		JMV		NFb		JNFb		LGI		LGI-P
		n^c	MPN (x10⁴)	n	MPN (x10⁴)	n	MPN(x10⁴) (x104) (x10⁴) (x10⁴)	n	MPN (x10⁴) (x104)	n	MPN (x10⁴)	n	MPN (x10⁴)
Soil	SF	5	7.67 ± 10.8	0	0	3	3.33 ± 3.5	0	0	0	0	8	11.00
	CH	0	0	0	0	5	7.67 ± 6.4	0	0	0	0	5	7.67
	ORG	10	16.00 ± 16.6	0	0	2	1.33 ± 2.3	1	1.33 ± 2.3	0	0	13	18.67
Tomato	SF	7	18.00 ± 23.8	2	3.00 ± 5.2	2	3.33 ± 0.6	2	2.00 ± 1.7	1	1.00 ± 1.7	14	27.33
	CH	9	9.00 ± 2.0	2	2.67 ± 2.3	4	4.00 ± 0.1	3	4.00 ± 0.1	0	0	18	19.67
	ORG	5	8.00 ± 3.6	0	0	5	6.33 ± 3.1	0	1.00 ± 1.7	0	0	10	15.33
Lulo	SF	5	10.0 ± 4.6	1	1.00 ± 1.7	4	8.33 ± 1.2	1	3.33 ± 0.6	1	2.67 ± 2.3	12	25.33
	CH	2	3.67 ± 0.6	0	1.00 ± 1.7	1	2.33 ± 2.1	1	2.33 ± 4.0	0	0	4	9.33
	ORG	6	5.67 ± 2.9	1	1.00 ± 1.7	5	7.67 ± 6.4	5	9.00 ± 6.0	0	1.33 ± 2.3	17	24.67
	Total n^d	49		6		31		13		2		101

^aSF, secondary forest soil with no agricultural use; CH, horticulture soil under conventional management; ORG, horticulture soil under organic management.

^bThe total population count is considered the sum of the MPNs from the semisolid N-free culture media for each sample source.

^cNumber of isolates retrieved.

^dTotal number of isolates obtained for each semisolid N-free culture medium considering all sample sources.