Fig 8. Evolutionary relationships of the newly-identified hematozoa viral sequences (MaRNAV-1 and MaRNAV-2).

(A) Phylogeny of all the newly-identified viral sequences. Red box: P. vivax viruses MaRNAV-1 (human or mosquitoes infection stage). Pink box: Leucocytozoon sp. MaRNAV-2 (bird infection stage). MaRNAV-1 viruses identified from P. vivax samples from this study are highlighted in red. Putative protozoan hosts are coloured depending on their belonging to the Alveolates (orange dark), Stramenopiles (light orange) and Euglenozoa (blue) major eukaryotic groups. Numbers indicate the branch support from 1000 bootstrap replicates. The virus tree is mid-point rooted for clarity only. (B) Eukaryotic host evolution and timescale, adapted from [38]. The two major groups Alveolates (red) and Euglenozoa (blue) are basal and their separation potentially occurred approximately two billion years ago [38].