Fig 9. Activated human microglia/HIV induce neuronal damage.

LUHMES-derived neurons were co-cultured with hμglia clone C20 or mixed population of cells that had been infected with HIV (HC20). Top: The neurons or co-cultures were stained with anti-beta-TUJ antibody (Red). Bottom: Neurons were co-cultured with C20 or HC20 cells and stained with anti-MAP2 (Red). Green: GFP expression in activated HC20 cells. Red: Alexa Fluor 488 antibodies were used as secondary antibodies. Blue: DAPI stained cell nuclei.