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. Author manuscript; available in PMC: 2020 Mar 20.
Published in final edited form as: J Am Chem Soc. 2019 Mar 7;141(11):4678–4686. doi: 10.1021/jacs.8b13610

Figure 2.

Figure 2.

Stopped-flow optical spectroscopy experiments monitoring formation of the PmoD CuA site at room temperature. The optical spectra were monitored over 1000 s after mixing 400 μM CuSO4 with 200 μM PmoD for (A) WT protein, (B) Cys65Ser variant, (C) Cys41Ser variant, (D) Met49Ala variant, (E) His51Ala variant, and (F) Cys41Ser/Cys65Ser variant. Red, 0.014 s-1.009 s; orange, 1.168-3.295 s; yellow, 3.634 s-8.183 s; green, 8.908 s-18.638 s; light blue, 20.184 s-40.976 s; blue, 44.29 s-190.867 s; purple, 206.014 s-409.204 s; dark purple, 441.588 s-1000 s.