Table 1.

Physicochemical characteristics of the prepared Chi/siRNA complexes.

C/N/P (molar ratio)	Ave DH (nm)	Ave PDI	Ave ξ (mV)	siRNA loading efficiency (%)
0/20/1	195.6 ± 2.55	0.38 ± 0.023	13.3 ± 2.00	83.53 ± 1.85
10/20/1	165.8 ± 2.80***	0.24 ± 0.011	−18.0 ± 0.40	81.81 ± 1.70
20/20/1	169.3 ± 4.42***	0.29 ± 0.033	−20.5 ± 0.99	83.34 ± 1.29
40/20/1	169.4 ± 5.39**	0.19 ± 0.015	−21.7 ± 3.43	83.50 ± 1.37
0/40/1	174.3 ± 7.72	0.26 ± 0.013	13.9 ± 1.44	89.15 ± 1.35
10/40/1	186.4 ± 5.66	0.19 ± 0.015	−16.4 ± 0.15	91.75 ± 0.95
20/40/1	184.6 ± 13.1	0.28 ± 0.015	−18.8 ± 0.21	90.02 ± 1.15
40/40/1	189.6 ± 3.71	0.16 ± 0.011	−24.3 ± 1.06	88.98 ± 1.24
DF-siRNA	192.0 ± 7.42	0.31 ± 0.042	48.6 ± 3.53	89.69 ± 1.06

Statistical analysis comparing HA coated Chi/siRNA complexes to the non-coated Chi/siRNA complexes.

^**p<0.01;

^***p<0.001.