Figure 19.

Gel (20% acrylamide, 7 M urea) image of a representative kinetics experiment with Klenow fragment exo-, 4CIN-TP (incorporating opposite template T), Cy5 labeled primer. The data show four increasing 4CIN-TP concentrations (right to left, 100, 250, 500, and 1000 μM) and time course for each concentration (5 points per concentration). Where the first (lane 1, left) is the reaction prior to initiation with 4CIN-TP followed by 4 time points of 30 s each for final timepoint at 2 min. The primer substrate band is the lower band, and the “extended” band is the higher band, indicating a larger DNA has been synthesized which incorporated 4CIN-TP.