Figure 6. Rad21-T262 phosphorylation modulates Mis4 binding to G1 chromosomes.

(A) DNA content analysis. Cultures of the indicated strains were shifted to 36.5°C to induce the cdc10-129 arrest and cells collected for ChIP after 3.5 hr. (B,C) Mis4-GFP ChIP relative to wild-type. Bars indicate mean ± SD, n = 4. ***p≤0.001, **p≤0.01, *p≤0.05, by two-tailed, unpaired t-test with 95% confidence interval (Figure 6—source data 1).