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Quantitative rtPCR analysis of relative TNF-α mRNA levels. Tissue was harvested (A) immediately, (B) after 24 hours of reperfusion, or (C) 5 days of reperfusion. Elevated TNF-α mRNA levels indicate increased gene expression relative to animals not subject to ischemia. Data are presented as mean ± S.E.M. N = 4 lymph nodes per group. *p<0.05, **p<0.01, ***p<0.001.
