Table 1.
Gene | Primer Sequence (5ʹ→3ʹ) | Amplicon Size (bp) | Annealing Temperature (°C) |
---|---|---|---|
SIM | F- TACAAGGGATTCGGCATCG R-TAATGGCCTGTTCCCATGTG |
741 | 60 |
IMP | F-GAAGGCGTTTATGTTCATAC R-GTATGTTTCAAGAGTGATGC |
587 | 53 |
GIM | F- CCTGTAGCGTTGCCAGCTTTA R- CAGCCCAAGAGCTAATTGAGG |
562 | 55 |
VIM | F-GTTTGGTCGCATATCGCAAC R-AATGCGCAGCACCAGGATAG |
382 | 55 |
SPM | F- CTGCTTGGATTCATGGGCGC R- CCTTTTCCGCGACCTTGATCG |
784 | 55 |
OprD2 | F-CGCCGACAAGAAGAACTAGC R-GTCGATTACAGGATCGACAG |
1332 | 55 |